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Source: http://www.doksinet Animal and Plant Health Inspection Service Veterinary Services September 2014 Swine Enteric Coronavirus Disease Testing Summary Report Summary Report for NAHLN Laboratory Testing April 2013 – June 2014 (Prior to the USDA Federal Order) Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Table of Contents Section 1. Introduction 3 1.1 1.2 1.3 1.4 Purpose of this Report .3 Background .3 Data-Sharing Participants.4 About the Data in this Report .4 Section 2. SECD Testing Overview 6 2.1 Testing Summary.6 Section 3. Porcine Epidemic Diarrhea Virus (PEDV) Testing Summary 7 3.1 PEDV Time Trends .7 3.2 PEDV Age Class Trends .10 3.3 PEDV Summary by State Figure 3. Maps showing the number of positive PEDV accessions from each State at intervals prior to the Federal Order on June 4, 2014 .12 Section 4. Porcine Deltacoronavirus (PDCoV) Testing Summary 16 4.1 PDCoV Time Trends Table 6.

Biological accessions and biological samples tested for PDCoV and number and percent positive by month. 16 4.2 PDCoV Age Class Trends .18 4.3 PDCoV Summary by State .19 Section 5. Environmental Testing Summary 21 Section 6. Appendices 23 This report was prepared by USDA-APHIS-VS Center for Epidemiology and Animal Health. For more information, contact: Dr. Brian McCluskey, VS Chief Epidemiologist, BrianJMcCluskey@aphisusdagov United States Department of Agriculture Animal and Plant Health Inspection Service Veterinary Services 2 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Section 1. Introduction 1.1 Purpose of this Report This report summarizes swine enteric coronavirus diseases (SECD) testing results that were voluntarily provided by National Animal Health Laboratory Network (NAHLN) laboratories prior to issuance of the June 2014 Federal Order (Reporting, Herd Monitoring, and Management of Novel

Swine Enteric Coronavirus Disease, June 5, 2014). 1.2 Background Porcine epidemic diarrhea virus (PEDV) was first detected in the United States in the spring of 2013, and NAHLN diagnostic laboratories began collating and sharing their PEDV testing data among themselves and with swine health stakeholders on April 16, 2013. In mid-June 2013, the USDA NAHLN Program Office began to assist with this data coordination effort. Starting the week of June 16, 2013, NAHLN laboratories began voluntarily providing their PEDV testing data to the NAHLN Program Office; this cooperative effort was intended to facilitate the collation of U.S PEDV testing information from NAHLN laboratories and sharing this information with key swine health stakeholders. The NAHLN Program Office started producing weekly reports summarizing PEDV testing on June 16, 2013. The reports provided a basic overview of the number of laboratory accessions tested and the number of positive accessions. Reports also tracked the

numbers of accessions tested and positives in each State and in different production types/age classes. In March 2014, labs began voluntarily providing their testing results for a second newly detected coronavirus, porcine deltacoronavirus (PDCoV). The reports incorporated testing information for both diseases and were referred to as Swine Enteric Coronavirus Disease (SECD) reports. Premises identification data are essential in an outbreak to determine the number of affected herds, areas affected, and other information that enables understanding and management of the disease situation. Because premises identification information was not provided to USDA with the SECD laboratory testing results and because of the suspected occurrence of repeat testing on affected herds, it was not possible to use the NAHLN laboratory accession data to accurately determine the number of infected herds/premises. Although the number of positive accessions could be counted and summarized, it was unknown

how many accessions represented repeat testing on the same herd. For example, if there were 100 positive accessions from State X, without premises identification information, it was impossible to determine if this represented 100 tests on a single herd or 100 different affected herds in that State. These data could be interpreted to represent anywhere from 1 to 100 affected herds. In response to the significant impact novel SECDs were having on the U.S pork industry, USDA issued a Federal Order on June 5, 2014. The Federal Order mandated reporting of SECD cases and specifically required that premises information be provided for SECD cases United States Department of Agriculture Animal and Plant Health Inspection Service Veterinary Services 3 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 to allow accurate assessment of the spread of these diseases and to facilitate disease control. 1.3 This report

summarizes the SECD testing information that was provided to VS prior to the Federal Order (prior to June 5, 2014). The report summarizes the number of laboratory accessions that were tested and found positive, but because of the reasons explained above, the accessions counts do not equate to herd/premises counts. Data-Sharing Participants Veterinary diagnostic laboratories that voluntarily reported PEDV and PDCoV testing data to the NAHLN Program office were: • Arkansas Livestock & Poultry Commission-Veterinary Diagnostic Laboratory • Athens Veterinary Diagnostic Laboratory, University of Georgia • University of Illinois Veterinary Diagnostic Laboratory • Illinois Department of Agriculture, Galesburg Animal Disease Laboratory • Iowa State University Veterinary Diagnostic Laboratory • Kansas State Veterinary Diagnostic Laboratory • Michigan State University-- Diagnostic Center for Population and Animal Health • University of Minnesota Veterinary Diagnostic

Laboratory • Veterinary Medical Diagnostic Laboratory, University of Missouri • University of Nebraska Veterinary Diagnostic Center • USDA National Veterinary Services Laboratories • Rollins Diagnostic Laboratory, North Carolina Department of Agriculture • Veterinary Diagnostic Laboratory, North Dakota State University • Ohio Animal Disease Diagnostic Laboratory - Ohio Department of Agriculture • Oregon State University Veterinary Diagnostic Laboratory • Indiana Animal Disease Diagnostic Laboratory, Purdue University • Animal Disease Research & Diagnostic Laboratory, South Dakota State University • Texas Veterinary Medical Diagnostic Laboratory, Texas A&M University 1.4 This report does not include data from testing conducted at private diagnostic laboratories or for research purposes. About the Data in this Report Data in this report were compiled from PEDV and PDCOV testing results data that were voluntarily provided by the above NAHLN laboratories.

Important caveats about these testing data: • As described previously, in this outbreak situation the number of accessions does not equate to the number of herds/premises. Because premises identification information were not provided by laboratories or by those who submitted the samples for testing, and because of the high occurrence of repeat testing on a herd in an outbreak, it was not possible to use NAHLN accession data to determine the number of infected herds/premises or accurately monitor disease spread. United States Department of Agriculture Animal and Plant Health Inspection Service Veterinary Services 4 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 • • • • • • • • • • A laboratory accession is a set of samples collected at a single premises on a single day and received at the laboratory. A laboratory accession represents a swine herd and samples within the

accession represents an individual animal tested for PEDV or PDCoV on a given date. Because multiple swine within a herd are often simultaneously infected, analyses often use the laboratory accession (rather than individual sample) as the epidemiological unit of interest. A single accession can include samples from multiple age classes or samples tested in different weeks. Therefore, the same accession can be counted in more than one age class category or more than one testing week summary. The collection site State data provided in this report are based on information provided by the testing laboratory. VS notified State animal health officials when a positive accession was reported for the first time in a State. Prior to the Federal Order, however, VS did not officially confirm positive accessions. Data in this report include PCR test results only. Some NAHLN laboratories also provided results data from different laboratory tests (e.g, IHC, IFA, ELISA), but those results are not

presented here. Data from feed samples are not included in this report. From mid-April to mid-June 2013, SECD testing data were compiled by the Iowa State University Veterinary Diagnostic Laboratory; only summary data on positive samples were provided for that time period. Starting the week of June 16, 2013, NAHLN laboratories began providing more granular data about PEDV testing to the USDA NAHLN Program Office; this allowed more information to be reported starting in mid-June. During the October 2013 federal government shutdown, the University of Minnesota Diagnostic Laboratory collected and reported PEDV testing data (weeks of Sept. 22 through Oct. 6, 2013) In November 2013, NAHLN laboratories began reporting data on PEDV-negative cases, thereby allowing reporting on the numbers of samples and accessions that were tested in addition to the number that were positive. Summaries “total number tested” and “percent positive” were calculated for samples tested after November 1,

2013. For NAHLN laboratory reporting, age classes were defined as suckling (< 1 month old or still on sow), nursery (1 month up to 3 months), grower/finisher (3 months up to 8 months), and sow/boar (8 months or older). United States Department of Agriculture Animal and Plant Health Inspection Service Veterinary Services 5 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Section 2. SECD Testing Overview 2.1 Testing Summary Table 1. Summary of testing and results for PEDV and PDCoV, April 2013 through June 4, 2014 (biological accessions and PCR testing only). For PEDV, data are summarized from April 15, 2013, through June 4, 2014; numbers of accessions tested were reported starting November 2013; percent positive was calculated from November 1, 2013 through June 4, 2014. For PDCoV, data are summarized from March 23, 2014, through June 4, 2014. PEDV PDCOV Number of Positive Accessions 6,976 260

Number of Accessions Tested 21,228 2,172 Percent Positive Accessions 32.9% 12.0% Number of States with Positive Accessions 30 15 Number of States with Accessions Tested 40 29 Number of NAHLN Labs that Provided Data 13 4 145,122 11,682 Number of Samples Tested / Result Records Processed United States Department of Agriculture Animal and Plant Health Inspection Service Veterinary Services 6 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Section 3. Porcine Epidemic Diarrhea Virus (PEDV) Testing Summary 3.1 PEDV Time Trends Table 2. Biological accessions and samples tested for PEDV and percent positive by month Biological Accessions Biological Samples Month Tested Positive % Pos Tested Positive April 2013 3 May 2013 112 June 2013 187 327 July 2013 113 557 Aug 2013 138 425 Sept 2013 134 458 Oct 2013 267 1,150 Nov 2013 1,064 414 38.9% 3,850 1,460 Dec 2013 2,294 630 27.5% 9,373 2,007 Jan 2014

2,774 953 34.4% 11,172 3,168 Feb 2014 3,650 1,228 33.6% 16,638 4,256 March 2014 3,601 1,123 31.2% 15,387 3,634 April 2014 3,775 1,053 27.9% 22,369 4,704 May 2014 3,590 757 21.1% 26,475 3,925 June 2014 489 84 17.2% 3,920 489 Total 21,228 6,976 29.4% 109,184 26,560 % Pos 37.9% 21.4% 28.4% 25.6% 23.6% 21.0% 14.8% 12.5% 21.7% Table 3. Biological accessions and samples tested for PEDV and number and percent positive by week Biological Accessions Biological Samples Week Tested Positive % Pos Tested Positive Apr 15, 2013 2 Apr 22, 2013 1 Apr 29, 2013 9 May 6, 2013 17 May 13, 2013 10 May 20, 2013 44 May 27, 2013 32 June 3, 2013 55 June 10, 2013 48 June 16, 2013 47 163 June 23, 2013 37 164 June 30, 2013 10 58 July 7, 2013 34 171 July 14, 2013 33 140 July 21, 2013 24 117 July 28, 2013 31 121 Aug 4, 2013 27 69 Aug 11, 2013 36 163 % Pos - United States Department of Agriculture Animal and Plant Health Inspection Service Veterinary Services 7 Source: http://www.doksinet SECD Testing

Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Week Aug 18, 2013 Aug 25, 2013 Sept 1, 2013 Sept 8, 2013 Sept 15, 2013 Sept 22, 2013 Sept 29,2013 Oct 6, 2013 Oct 13, 2013 Oct 20, 2013 Oct 27, 2013 Nov 3, 2013 Nov 10, 2013 Nov 17, 2013 Nov 24, 2013 Dec 1, 2013 Dec 8, 2013 Dec 15, 2013 Dec 22, 2013 Dec 29, 2013 Jan 5, 2014 Jan 12, 2014 Jan 19, 2014 Jan 26, 2014 Feb 2, 2014 Feb 9, 2014 Feb 16, 2014 Feb 23, 2014 Mar 2, 2014 Mar 9, 2014 Mar 16, 2014 Mar 23, 2014 Mar 30, 2014 Apr 6, 2014 Apr 13, 2014 Apr 20, 2014 Apr 27, 2014 May 4, 2014 May 11, 2014 May 18, 2014 May 25, 2014 June 1, 2014 Total Tested 219 221 239 338 565 572 501 418 439 693 497 816 576 917 897 916 927 846 949 835 831 926 889 825 798 824 856 855 811 756 489 21,228 Biological Accessions Positive % Pos 30 26 31 28 32 40 41 43 57 68 84 90 41% 92 42% 113 47% 96 28% 139 25% 132 23% 185 37% 118 28% 122 28% 188 27% 215 43% 218 27% 267 46% 295 32% 306 34% 315 34% 313 34% 281 33% 295 31% 270 32% 247

30% 260 28% 272 31% 222 27% 211 26% 198 24% 192 22% 190 22% 158 19% 143 19% 84 17% 6,976 33% United States Department of Agriculture Tested 649 696 741 1,617 2,481 2,356 1,816 1,773 2,077 2,876 1,735 3,386 2,073 4,025 3,901 4,374 4,317 3,351 3,732 3,601 4,112 5,221 4,859 4,645 5,107 5,350 5,719 6,398 6,106 6,023 6,306 109,184 Animal and Plant Health Inspection Service Biological Samples Positive 72 71 85 79 130 156 170 196 252 288 331 292 373 304 412 367 451 524 409 546 590 621 816 851 934 1,060 1,201 1,068 751 851 999 886 1,279 1,104 943 941 858 978 949 821 896 757 26,560 Veterinary Services % Pos 45% 54% 41% 25% 15% 19% 29% 23% 26% 21% 36% 24% 41% 23% 27% 27% 25% 22% 23% 28% 22% 24% 23% 20% 18% 16% 17% 15% 13% 15% 12% 24% 8 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Figure 1A. Number of biological accessions tested for PEDV and number of positive accessions by week Numbers of accessions tested were

reported starting November 2013. 1000 Number of Accessions 900 Accessions Tested Positive Accessions 800 700 600 500 400 300 200 100 0 Figure 1B. Percentage of biological accessions that were positive for PEDV Numbers of accessions tested were reported starting November 2013; percent positive was calculated from November 1, 2013 through June 4, 2014. Percent Positive Accessions 100.0% % of Accessions Positive for PEDV 80.0% 60.0% 40.0% 20.0% 0.0% United States Department of Agriculture Animal and Plant Health Inspection Service Veterinary Services 9 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 3.2 PEDV Age Class Trends Table 4. PEDV Summary by Age Class Biological accessions tested for PEDV and the number positive and percent positive for each farm type/age class by month. Suckling Nursery Grower / Finisher Sow / Boar Unk Month Tested Pos % Pos Tested Pos % Pos Tested Pos % Pos Tested Pos %

Pos Tested Pos % Pos Apr 2013 0 0 2 0 0 May 2013 0 0 70 23 8 Jun 2013 14 12 84 36 35 Jul 2013 13 24 40 20 20 Aug 2013 57 31 19 25 12 Sep 2013 39 31 28 20 20 Oct 2013 89 47 72 39 23 Nov 2013 146 89 61.0% 228 105 461% 220 127 577% 61 25 41.0% 437 79 18.1% Dec 2013 260 118 45.4% 432 138 319% 589 171 290% 154 40 260% 909 177 19.5% Jan 2014 350 187 53.4% 450 190 422% 543 183 337% 168 65 387% 1,353 356 263% Feb 2014 488 248 50.8% 609 228 374% 601 154 256% 285 110 386% 1,788 530 296% Mar2014 515 261 50.7% 540 186 344% 530 118 223% 309 90 291% 1,861 505 271% Apr 2014 534 229 42.9% 512 177 346% 589 121 205% 362 61 169% 1,924 486 253% May 2014 568 199 35.0% 511 119 233% 595 107 180% 413 61 148% 1,663 301 181% Jun 2014 82 22 26.8% 61 11 18.0% 77 10 13.0% 46 5 10.9% 236 36 15.3% Total 2,941 1,565 46.0% 3,341 1,298 345% 3,743 1,176 265% 1,797 573 254% 10,168 2,567 243% *Number tested was reported starting November 2013; percent positive is calculated using data from Nov. 1, 2013, through current

week. Figure 2A. Percentage of biological accessions tested for PEDV and percent positive by farm type/age class Numbers of accessions tested were reported starting November 2013; percent positive was calculated from November 1, 2013 through June 4, 2014. United States Department of Agriculture Animal and Plant Health Inspection Service Veterinary Services 10 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Figure 2B. Percentage of PEDV positive accessions for each farm type/age class Numbers of accessions tested were reported starting November 2013; percent positive was calculated from November 1, 2013 through June 4, 2014. United States Department of Agriculture Animal and Plant Health Inspection Service Veterinary Services 11 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 3.3 PEDV Summary by State Figure 3. Maps showing

the number of positive PEDV accessions from each State at intervals prior to the Federal Order on June 4, 2014 United States Department of Agriculture Animal and Plant Health Inspection Service Veterinary Services 12 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 United States Department of Agriculture Animal and Plant Health Inspection Service Veterinary Services 13 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Table 5. Number of PEDV positive biological accessions in each State, by week Total Positive Accessions AZ CA CO IA ID IL IN KS KY MD MI MN 1 10 6 19 Combined ISU data, 4/16/13-6/15/13 218 7 102 3 6/16/2013 47 1 10 1 7 8 6/23/2013 37 1 15 1 1 4 6/30/2013 10 7/7/2013 34 1 6 7/14/2013 33 2 7/21/2013 24 7/28/2013 MO MS MT NC ND NE NY OH OK PA SC SD TN TX VA VT WI WY UNK 1 2 3

38 4 2 2 3 2 3 2 11 3 2 7 7 4 4 7 4 7 1 6 1 3 31 1 5 1 1 7 3 2 8/4/2013 27 1 9 1 1 6 1 2 5 8/11/2013 36 6 5 13 5 7 8/18/2013 30 1 6 1 5 1 2 14 8/25/2013 26 2 1 3 2 4 1 10 3 9/1/2013 31 6 1 8 1 4 4 5 9/8/2013 28 9/15/2013 32 9/22/2013 40 9/29/2013 1 7 1 6 13 2 2 1 4 1 6 1 1 1 10 1 13 5 1 7 1 3 2 12 1 3 1 5 1 24 4 41 2 5 2 2 19 6 1 10/6/2013 43 1 6 6 1 1 21 6 1 10/13/2013 57 10 4 1 6 21 13 2 10/20/2013 68 1 2 22 9 1 10/27/2013 5 17 1 13 2 1 3 1 1 1 1 1 1 20 2 1 9 84 32 4 1 3 11/3/2013 90 38 1 1 6 2 7 2 14 5 10 1 11/10/2013 92 1 51 1 2 8 1 15 4 5 1 11/17/2013 113 1 47 17 12 4 11 1 11/24/2013 96 1 25 19 19 9 12 12/1/2013 139 2 65 23 8 5 6 Veterinary Services 14 United States Department of Agriculture 1 1 2 5 10 3 5 4 1 9 4 Animal and Plant Health Inspection

Service 1 1 1 3 3 1 1 3 1 6 3 2 1 1 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Total Positive Accessions AZ CA CO IA 65 1 ID IL 8 IN 6 KS KY MD MI 5 2 96 15 8 3 MN 18 12/8/2013 132 12/15/2013 185 12/22/2013 118 1 61 8 3 5 12/29/2013 122 1 49 13 2 4 1/5/2014 188 3 81 10 2 9 1 1 46 1/12/2014 215 1 88 19 7 7 3 4 31 1/19/2014 218 2 70 21 9 10 1 4 1/26/2014 267 2 87 39 24 3 1 2/2/2014 295 2 93 27 15 3 2/9/2014 306 2 91 27 22 7 2/16/14 315 3 90 35 25 7 2/23/14 313 2 79 41 22 1 3/2/14 281 1 1 81 32 26 3/9/14 295 2 7 71 43 3/16/14 270 2 54 3/23/14 247 2 3/30/13 260 1 4/6/14 272 4/13/14 222 4/20/14 MO MS MT NC ND NE NY OH OK PA SC SD TN TX VA VT WI WY UNK 3 12 3 7 1 1 1 27 5 19 1 3 5 17 2 5 1 5 3 20 4 17 2 5 15 1 7 4 4 20 5 9 8 51 7 19 2 10 2 50 7 15

7 1 4 76 9 24 4 1 9 62 4 23 9 7 59 4 24 10 61 8 23 6 3 47 11 22 13 13 52 44 21 5 16 48 43 14 5 1 51 41 17 9 2 5 48 43 17 1 5 42 43 211 2 52 4/27/14 198 1 44 5/4/14 192 5/11/14 190 5 5/18/14 158 5/25/14 6/1/14 Total 2 1 1 2 2 1 1 1 4 2 2 3 2 2 3 11 4 4 3 2 1 1 3 11 4 4 3 1 1 1 9 21 6 3 4 2 1 2 9 6 22 9 7 5 1 2 2 1 6 12 19 5 12 3 1 2 2 7 26 2 19 1 3 6 5 7 16 5 1 13 9 3 7 6 5 40 17 17 6 1 17 9 5 6 2 3 9 45 16 20 9 16 5 1 5 1 17 48 8 24 7 17 5 1 5 2 4 7 10 49 12 23 10 22 10 2 2 3 5 16 6 9 46 7 20 2 10 4 2 4 30 12 13 6 36 9 9 12 9 9 3 3 1 5 32 15 11 40 5 16 7 12 6 5 1 2 25 8 3 9 33 17 21 6 7 10 1 5 4 31 31 10 4 1 10 35 14 18 4 7 8 3 4 3 3 32 25 11 1 1 3 27 5 23 9 3 4 1 6 143 3 32 26 5 3 6 23 8 15 10 1 4 2 4 84 2 9

11 5 2 5 16 9 13 4 7,178 1 1 1 1 3 40 5 1 1 1 1 1 1 1 1 11 91 2,075 4 758 388 266 18 1 180 1,189 205 1 1 1 1 719 1 1 143 1 2 1 3 4 5 322 400 89 1 2 1 1 1 3 3 1 1 1 9 2 5 1 1 2 1 1 3 1 1 3 2 93 13 80 1 1 1 18 9 89 *A single accession may include samples tested over more than one week; total accessions is calculated as the distinct count of accessions tested rather than the sum of accessions tested by week. United States Department of Agriculture Animal and Plant Health Inspection Service Veterinary Services 15 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Section 4. Porcine Deltacoronavirus (PDCoV) Testing Summary 4.1 PDCoV Time Trends Table 6. Biological accessions and biological samples tested for PDCoV and number and percent positive by month. Biological Accessions Biological Samples Month Tested Positive % Pos Tested Positive % Pos Mar

2014 45 7 15.6% 150 10 6.7% Apr 2014 947 124 13.1% 4,095 382 9.3% May 2014 1,042 112 10.7% 4,858 337 6.9% Jun 2014 140 15 10.7% 660 42 6.4% Total 2,172 260 12.0% 9,763 771 7.9% Table 7. Biological accessions and biological samples tested for PDCoV and number and percent positive by week. Biological Accessions Biological Samples Week Tested Positive % Pos Tested Positive % Pos 3/23/2014 2 0% 5 0% 3/30/2014 177 25 14% 824 92 11% 4/6/2014 241 32 13% 970 100 10% 4/13/2014 220 29 13% 856 93 11% 4/20/2014 215 28 13% 884 65 7% 4/27/2014 256 33 13% 1145 102 9% 5/4/2014 253 41 16% 1358 115 8% 5/11/2014 228 23 10% 1140 68 6% 5/18/2014 236 19 8% 986 45 5% 5/25/2014 213 15 7% 935 49 5% 6/1/2014 140 15 11% 660 42 6% Total 2,172 260 12% 9,763 771 8% United States Department of Agriculture Animal and Plant Health Inspection Service Veterinary Services 16 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Figure 4A. Number

of biological accessions tested for PDCoV and number of positive accessions by week 1000 Number of Accessions 900 Accessions Tested Positive Accessions 800 700 600 500 400 300 200 100 0 Figure 4B. Percentage of biological accessions that were positive for PDCoV Percent Positive Accessions 100% 80% 60% 40% 20% 0% United States Department of Agriculture Animal and Plant Health Inspection Service Veterinary Services 17 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 4.2 PDCoV Age Class Trends Table 8. PDCoV Summary by Age Class Biological accessions tested for PDCoV and the number positive and percent positive for each farm type / age class by month. Suckling Nursery Grower / Finisher Sow / Boar Unk Month Tested Pos % Pos Tested Pos % Pos Tested Pos % Pos Tested Pos % Pos Tested Pos % Pos Mar2014 12 1 8.3% 10 1 10.0% 2 0 0.0% 2 1 50.0% 21 4 19.0% Apr 2014 195 18 9.2% 141 25 177% 75 7 9.3% 95 11

11.6% 477 69 145% May 2014 198 12 6.1% 143 17 119% 107 13 121% 93 8 8.6% 543 67 123% Jun 2014 24 1 4.2% 10 1 10.0% 11 2 18.2% 16 1 6.3% 81 11 13.6% Total 428 32 7.5% 304 44 145% 195 22 113% 206 21 102% 1,121 151 135% Figure 5a. Percentage of biological accessions tested for PDCoV and percent positive by farm type/age class United States Department of Agriculture Animal and Plant Health Inspection Service Veterinary Services 18 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Figure 5B. Percentage of PDCoV positive accessions for each farm type/age class Numbers of accessions tested was reported starting November 2013; percent positive was calculated from November 1, 2013 through June 4, 2014 4.3 PDCoV Summary by State Table 9. Positive PDCoV biological accessions for each State, by week Total Testing Positive Week Accessions IA IL IN KS MI MN MO MT 3/30/14 25 3 4 1 4 6 1 4/6/14 32 4 2 7 2 5 4/13/14 29 5

8 4 1 7 4/20/14 28 2 3 3 10 1 4/27/14 33 3 7 3 1 1 11 1 5/4/14 41 3 10 5 2 6 1 5/11/14 23 2 2 3 8 5/18/14 19 2 3 1 5 5/25/14 15 5 2 4 6/1/14 22 3 4 1 5 1 Total 258 22 45 34 2 11 63 3 1 NC NE 1 1 1 1 4 1 2 2 2 1 1 9 8 OH 6 7 1 3 2 5 3 3 2 4 34 OK PA SD TX UNK 1 1 3 1 1 1 1 1 1 1 1 7 1 1 1 2 3 2 10 1 1 1 2 A single accession may include samples tested over more than one week; total accessions is calculated as the distinct count of accessions tested rather than the sum of accessions tested by week. United States Department of Agriculture Animal and Plant Health Inspection Service Veterinary Services 19 1 6 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Figure 6. Maps showing the number of positive PDCoV accessions from each State at intervals prior to the Federal Order on June 4, 2014. United States Department of Agriculture Animal and Plant Health Inspection Service Veterinary

Services 20 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Section 5. Environmental Testing Summary Table 10. Environmental accessions tested for PEDV and PDCoV and percent positive by week PEDV - Environmental Accessions PDCoV - Environmental Accessions Week Tested PEDV Positive % Pos Tested PDCoV Positive % Pos June 16, 2013 17 7 41% June 23, 2013 19 13 68% June 30, 2013 16 12 75% July 7, 2013 13 13 100% July 14, 2013 15 7 47% July 21, 2013 19 4 21% July 28, 2013 11 4 36% Aug 4, 2013 7 5 71% Aug 11, 2013 11 1 9% Aug 18, 2013 20 2 10% Aug 25, 2013 12 3 25% Sept 1, 2013 8 3 38% Sept 8, 2013 21 3 14% Sept 15, 2013 41 6 15% Sept 22, 2013 47 6 13% Sept 29,2013 40 9 23% Oct 6, 2013 19 8 42% Oct 13, 2013 24 5 21% Oct 20, 2013 27 10 37% Oct 27, 2013 36 9 25% Nov 3, 2013 51 25 49% Nov 10, 2013 40 17 43% Nov 17, 2013 68 29 43% Nov 24, 2013 70 25 36% Dec 1, 2013 69 32 46% Dec 8, 2013 84 26 31% Dec 15, 2013 101 34 34%

Dec 22, 2013 56 18 32% Dec 29, 2013 42 15 36% Jan 5, 2014 77 32 42% Jan 12, 2014 84 43 51% Jan 19, 2014 116 38 33% Jan 26, 2014 82 37 45% Feb 2, 2014 175 77 44% Feb 9, 2014 117 35 30% Feb 16, 2014 142 66 46% Feb 23, 2014 144 55 38% Mar 2, 2014 119 45 38% Mar 9, 2014 160 64 40% Mar 16, 2014 133 51 38% Mar 23, 2014 150 54 36% United States Department of Agriculture Animal and Plant Health Inspection Service Veterinary Services 21 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Week Mar 30, 2014 Apr 6, 2014 Apr 13, 2014 Apr 20, 2014 Apr 27, 2014 May 4, 2014 May 11, 2014 May 18, 2014 May 25, 2014 June 1, 2014 Total PEDV - Environmental Accessions Tested PEDV Positive % Pos 144 61 42% 170 78 46% 151 54 36% 157 64 41% 180 66 37% 115 36 31% 139 42 30% 170 49 29% 122 36 30% 90 11 12% 3,941 1,445 37% United States Department of Agriculture PDCoV - Environmental Accessions Tested PDCoV Positive % Pos 22 6 27% 38 5

13% 39 2 5% 36 4 11% 31 5 16% 37 9 24% 36 3 8% 36 1 3% 23 1 4% 14 0 0% 312 36 12% Animal and Plant Health Inspection Service Veterinary Services 22 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Section 6. Appendices Appendix A. PEDV - Weekly Testing Data for PEDV by State Biological Accessions State AL AL AL AL AR AR AR AR AR AR AR AR AR AR AR AR AR AR AR AR AR AR AZ AZ AZ AZ AZ AZ AZ AZ AZ AZ AZ AZ AZ AZ Week Tested 11/24/2013 1/12/2014 3/16/2014 5/25/2014 TOTAL 11/10/2013 11/17/2013 12/8/2013 12/15/2013 12/22/2013 1/5/2014 1/19/2014 1/26/2014 2/23/2014 3/2/2014 3/9/2014 3/16/2014 3/23/2014 3/30/2014 4/6/2014 4/13/2014 4/20/2014 4/27/2014 TOTAL 1/26/2014 2/2/2014 2/16/2014 2/23/2014 3/2/2014 3/9/2014 3/16/2014 3/23/2014 3/30/2014 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 1 1 1 1 4 1 2 1 4 2 2 1 2 4 1 5 2 3 1 3 5 3 2 59 1 Positive 3 1 7 2 1 1 2 1 1 1 United States Department of Agriculture

0 0 1 1 3 % Pos Biological Samples Tested Positive Environmental Samples Tested Positive 0 0 0 0 7 0 14 14 18 12 0 3 2 2 25 20 20 6 11 5 40 37 7 12 29 5 0.00% 0.00% 0.00% 0.00% 0.00% 0.0% 0.0% 0.0% 0.0% 0.0% 0.0% 0.0% 0.0% 0.0% 0.0% 0.0% 0.0% 0.0% 0.0% 0.0% 0.0% 0.0% 0.0% 0.00% 100.00% 0 1 23 2 45 11 9 4 11 16 5 18 12 8 6 4 33 7 7 302 7 33.33% 0.00% 42.86% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 5 2 11 2 1 2 3 2 2 1 1 6 Animal and Plant Health Inspection Service Veterinary Services 23 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Biological Accessions State AZ AZ AZ AZ CA CA CA CA CA CA CA CA CA CA CA CA CA CA CA CA CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO Week Tested 5/11/2014 5/18/2014 5/25/2014 6/1/2014 TOTAL 12/22/2013 12/29/2013 1/12/2014 2/2/2014 2/9/2014 3/2/2014 3/9/2014 3/23/2014 3/30/2014 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/18/2014 5/25/2014

6/1/2014 TOTAL 6/16/2013 6/23/2013 6/30/2013 7/7/2013 7/14/2013 7/21/2013 7/28/2013 8/4/2013 8/11/2013 8/18/2013 8/25/2013 9/1/2013 9/8/2013 9/15/2013 9/22/2013 9/29/2013 10/6/2013 10/13/2013 10/20/2013 10/27/2013 11/3/2013 1 1 1 1 25 1 1 1 1 2 4 9 3 3 3 3 2 1 2 1 1 38 4 4 3 3 4 4 1 4 16 5 10 9 10 1 8 9 4 6 7 4 3 Positive 5 1 1 1 1 1 2 1 2 1 11 1 1 United States Department of Agriculture 1 2 1 1 1 6 1 2 1 2 1 1 % Pos 0.00% 0.00% 0.00% 0.00% 20.00% 100.00% 100.00% 100.00% 100.00% 0.00% 25.00% 22.22% 0.00% 33.33% 66.67% 33.33% 0.00% 0.00% 0.00% 0.00% 0.00% 28.95% 25.00% 25.00% 0.00% 33.33% 50.00% 25.00% 100.00% 25.00% 37.50% 20.00% 0.00% 22.22% 0.00% 0.00% 12.50% 22.22% 25.00% 0.00% 14.29% 0.00% 0.00% Biological Samples Tested Positive 1 1 1 1 42 2 1 1 4 2 6 13 6 14 50 4 2 2 2 1 2 112 23 15 38 15 75 26 20 32 46 23 78 101 37 3 29 56 14 17 28 13 11 Environmental Samples Tested Positive 35 16 14 2 1 1 2 242 91 1 4 4 2 4 2 19 2 6 4 6 6 4 2 12 5 4 70 2 0 4 1 16 4 16

26 1 5 2 1 Animal and Plant Health Inspection Service 28 31 73 41 2 1 2 8 16 Veterinary Services 24 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Biological Accessions State CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CT GA GA GA GA GA GA GA GA GA GA Biological Samples Tested Positive Week Tested Positive % Pos 11/10/2013 11/17/2013 11/24/2013 12/1/2013 12/8/2013 12/15/2013 12/22/2013 12/29/2013 1/5/2014 1/12/2014 1/19/2014 1/26/2014 2/2/2014 2/9/2014 2/16/2014 2/23/2014 3/2/2014 3/9/2014 3/16/2014 3/23/2014 3/30/2014 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 5/11/2014 5/18/2014 5/25/2014 6/1/2014 TOTAL 3/16/2014 TOTAL 6/23/2013 12/29/2013 2/9/2014 3/2/2014 3/16/2014 3/30/2014 4/6/2014 4/13/2014 4/27/2014 5/4/2014 4 4 7 7 3 7 1 3 8 6 5 5 7 4 8 5 8 14 3 2 1 9 6 6 14 3 7 7 5 6 294 1 1 1 1 1 2 25.00% 25.00% 14.29% 28.57% 0.00% 14.29% 0.00%

0.00% 37.50% 16.67% 40.00% 40.00% 28.57% 50.00% 37.50% 40.00% 12.50% 50.00% 66.67% 100.00% 100.00% 55.56% 83.33% 33.33% 7.14% 0.00% 71.43% 42.86% 60.00% 33.33% 29% 0.00% 0% 12 25 36 27 10 23 3 16 42 25 32 28 55 61 56 43 46 80 41 37 20 106 37 40 77 25 100 41 35 31 1910 1 1 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 3 4 1 2 8 4 4 4 4 1 3 1 2 2 2 2 3 2 1 7 2 2 1 5 5 2 1 5 3 3 2 84 3 1 1 1 2 1 1 1 1 United States Department of Agriculture 0 Environmental Samples Tested Positive 21 4 4 31 1 4 8 15 17 30 25 29 26 5 38 24 26 20 49 29 22 1 2 36 14 2 33 14 8 5 543 123 34 87 39 1056 22 5 36 1 215 0 0 5 0 2 15 7 5 Animal and Plant Health Inspection Service 35 10 43 4 19 13 8 19 45 71 37 64 1 14 8 2 37 12 25 31 Veterinary Services 25 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Biological Accessions State GA GA HI HI IA IA IA IA IA IA IA IA IA IA IA IA IA IA IA IA IA IA IA IA

IA IA IA IA IA IA IA IA IA IA IA IA IA IA IA IA IA Week Tested 5/11/2014 5/18/2014 TOTAL 3/16/2014 4/27/2014 TOTAL 6/16/2013 6/23/2013 6/30/2013 7/7/2013 7/14/2013 7/21/2013 7/28/2013 8/4/2013 8/11/2013 8/18/2013 8/25/2013 9/1/2013 9/8/2013 9/15/2013 9/22/2013 9/29/2013 10/6/2013 10/13/2013 10/20/2013 10/27/2013 11/3/2013 11/10/2013 11/17/2013 11/24/2013 12/1/2013 12/8/2013 12/15/2013 12/22/2013 12/29/2013 1/5/2014 1/12/2014 1/19/2014 1/26/2014 2/2/2014 2/9/2014 2/16/2014 2/23/2014 1 2 15 1 1 2 17 16 8 9 12 8 11 14 11 11 6 14 6 15 13 11 14 15 27 45 57 65 53 102 190 167 149 151 141 187 106 171 110 211 203 184 165 Positive 0 0 10 15 3 6 7 6 5 9 5 6 2 6 7 3 5 6 10 20 32 38 51 47 25 65 65 96 61 49 81 88 70 87 93 91 90 79 United States Department of Agriculture % Pos 0.00% 0.00% 0% 0.00% 0.00% 0% 58.82% 93.75% 37.50% 66.67% 58.33% 75.00% 45.45% 64.29% 45.45% 54.55% 33.33% 42.86% 0.00% 46.67% 23.08% 45.45% 42.86% 66.67% 74.07% 71.11% 66.67% 78.46% 88.68% 24.51% 34.21% 38.92% 64.43%

40.40% 34.75% 43.32% 83.02% 40.94% 79.09% 44.08% 44.83% 48.91% 47.88% Biological Samples Tested Positive 4 7 45 1 1 2 29 27 14 20 27 18 47 42 25 60 12 71 25 31 29 28 44 34 67 124 170 175 91 361 639 481 369 476 490 589 253 641 354 699 617 839 565 Environmental Samples Tested Positive 0 5 0 0 15 25 4 8 17 10 23 23 11 15 2 9 0 14 2 0 12 2 1 1 12 3 1 12 3 1 9 3 16 11 16 46 83 111 129 82 49 141 126 203 115 118 189 188 173 221 247 200 223 156 Animal and Plant Health Inspection Service 5 4 3 4 6 6 6 19 31 28 113 248 141 33 49 30 106 67 75 117 190 177 141 212 4 4 3 2 9 24 15 10 42 15 7 12 11 34 22 8 14 40 36 56 71 Veterinary Services 26 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Biological Accessions State IA IA IA IA IA IA IA IA IA IA IA IA IA IA ID ID ID ID ID ID ID IL IL IL IL IL IL IL IL IL IL IL IL IL IL IL IL IL IL IL IL Week Tested Positive % Pos 3/2/2014 3/9/2014 3/16/2014

3/23/2014 3/30/2014 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 5/11/2014 5/18/2014 5/25/2014 6/1/2014 TOTAL 2/2/2014 2/9/2014 2/23/2014 3/16/2014 3/23/2014 3/30/2014 5/4/2014 TOTAL 6/16/2013 6/23/2013 6/30/2013 7/7/2013 7/14/2013 7/21/2013 7/28/2013 8/4/2013 8/11/2013 8/18/2013 8/25/2013 9/1/2013 9/8/2013 9/15/2013 9/22/2013 9/29/2013 10/6/2013 10/13/2013 10/20/2013 10/27/2013 152 178 129 132 135 136 118 132 118 122 129 109 106 55 4446 1 3 1 1 1 1 2 10 4 7 2 9 5 5 12 5 9 11 15 3 3 6 6 7 4 9 13 11 81 71 54 48 51 48 42 52 44 40 31 32 32 9 1974 1 53.29% 39.89% 41.86% 36.36% 37.78% 35.29% 35.59% 39.39% 37.29% 32.79% 24.03% 29.36% 30.19% 16.36% 44% 100.00% 0.00% 100.00% 100.00% 0.00% 0.00% 50.00% 40% 0.00% 0.00% 0.00% 11.11% 0.00% 0.00% 8.33% 20.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 15.38% 36.36% United States Department of Agriculture 1 1 1 4 1 1 1 2 4 Biological Samples Tested Positive 511 690 510 658 652 639 577 840 543 610 762 628 755 337 17295

1 3 2 1 1 2 24 34 4 24 4 23 10 8 47 8 11 17 25 3 9 8 7 9 8 12 40 30 Environmental Samples Tested Positive 133 379 227 213 239 222 212 308 197 217 216 268 192 103 4970 27 75 44 55 81 42 23 88 50 21 22 34 31 1 1064 0 0 152 148 159 103 171 101 186 126 120 146 104 112 123 28 4796 1 2 1 17 21 4 6 1 8 7 17 23 Animal and Plant Health Inspection Service 2 Veterinary Services 27 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Biological Accessions State IL IL IL IL IL IL IL IL IL IL IL IL IL IL IL IL IL IL IL IL IL IL IL IL IL IL IL IL IL IL IL IN IN IN IN IN IN IN IN IN IN IN Week Tested Positive % Pos 11/3/2013 11/10/2013 11/17/2013 11/24/2013 12/1/2013 12/8/2013 12/15/2013 12/22/2013 12/29/2013 1/5/2014 1/12/2014 1/19/2014 1/26/2014 2/2/2014 2/9/2014 2/16/2014 2/23/2014 3/2/2014 3/9/2014 3/16/2014 3/23/2014 3/30/2014 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 5/11/2014 5/18/2014 5/25/2014

6/1/2014 TOTAL 6/16/2013 6/23/2013 7/7/2013 7/14/2013 7/21/2013 7/28/2013 8/4/2013 8/18/2013 8/25/2013 9/1/2013 9/8/2013 4 12 10 8 60 68 44 32 58 82 61 77 58 93 81 110 91 101 105 115 96 145 114 115 96 122 117 118 117 120 65 2641 1 2 2 5 1 1 3 1 2 1 1 1 25.00% 0.00% 50.00% 0.00% 16.67% 11.76% 34.09% 25.00% 22.41% 12.20% 31.15% 27.27% 67.24% 29.03% 33.33% 31.82% 45.05% 31.68% 40.95% 38.26% 44.79% 28.28% 37.72% 37.39% 31.25% 26.23% 21.37% 26.27% 21.37% 21.67% 16.92% 29% 100.00% 50.00% 100.00% 80.00% 100.00% 100.00% 33.33% 100.00% 50.00% 100.00% 0.00% 5 10 8 15 8 13 10 19 21 39 27 27 35 41 32 43 44 43 41 43 43 30 32 25 31 25 26 11 757 1 1 2 4 1 1 1 1 1 1 United States Department of Agriculture Biological Samples Tested Positive 7 15 20 32 235 245 146 161 352 377 256 330 269 503 365 426 424 344 387 550 655 934 845 881 826 1126 1009 1225 1102 1200 757 16311 1 3 3 11 4 1 9 3 3 1 1 Environmental Samples Tested Positive 30 4 13 9 15 33 31 13 138 106 10 19 12 12 25 17 20 30 45 27 21 17

72 42 125 101 45 89 115 93 114 1460 24 3 11 39 27 36 55 149 69 86 102 149 108 108 190 193 104 153 211 271 331 345 329 254 256 190 240 248 226 57 4591 1 2 3 8 4 1 1 2 2 1 Animal and Plant Health Inspection Service 1 7 6 1 1 20 3 1 2 3 2 6 3 5 4 15 15 31 38 7 18 16 29 3 261 Veterinary Services 28 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Biological Accessions State IN IN IN IN IN IN IN IN IN IN IN IN IN IN IN IN IN IN IN IN IN IN IN IN IN IN IN IN IN IN IN IN IN IN KS KS KS KS KS KS KS KS Week Tested Positive % Pos 9/15/2013 9/22/2013 10/20/2013 10/27/2013 11/3/2013 11/17/2013 11/24/2013 12/1/2013 12/8/2013 12/15/2013 12/22/2013 12/29/2013 1/5/2014 1/12/2014 1/19/2014 1/26/2014 2/2/2014 2/9/2014 2/16/2014 2/23/2014 3/2/2014 3/9/2014 3/16/2014 3/23/2014 3/30/2014 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 5/11/2014 5/18/2014 5/25/2014 6/1/2014 TOTAL 6/16/2013 6/23/2013 6/30/2013 7/7/2013

7/14/2013 7/21/2013 7/28/2013 8/4/2013 3 1 1 1 1 4 7 19 19 15 12 9 14 12 19 34 33 46 53 54 56 59 47 37 54 43 37 48 50 39 28 37 34 25 971 13 3 5 6 10 5 11 10 1 1 1 1 1 3 4 9 6 8 3 2 2 7 9 24 15 22 25 22 26 22 21 14 17 17 16 12 15 8 10 11 5 5 379 7 1 33.33% 100.00% 100.00% 100.00% 100.00% 75.00% 57.14% 47.37% 31.58% 53.33% 25.00% 22.22% 14.29% 58.33% 47.37% 70.59% 45.45% 47.83% 47.17% 40.74% 46.43% 37.29% 44.68% 37.84% 31.48% 39.53% 43.24% 25.00% 30.00% 20.51% 35.71% 29.73% 14.71% 20.00% 39.03% 53.85% 33.33% 0.00% 66.67% 70.00% 60.00% 63.64% 60.00% United States Department of Agriculture 4 7 3 7 6 Biological Samples Tested Positive 15 1 2 1 2 9 20 35 27 34 35 77 48 25 41 69 71 149 142 114 159 194 120 125 188 160 140 256 202 212 156 239 226 121 3455 93 12 19 75 56 71 84 36 Environmental Samples Tested Positive 1 3 1 1 1 9 5 1 1 2 1 2 6 5 19 9 15 4 3 2 8 13 46 27 47 55 43 53 49 36 25 47 41 41 36 41 31 43 47 14 16 854 37 4 47 35 38 50 19 Animal and Plant Health Inspection

Service 2 22 4 11 24 9 5 9 21 3 7 10 18 7 7 173 64 47 32 33 13 10 1 2 5 5 9 2 9 2 6 48 4 17 7 16 Veterinary Services 29 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Biological Accessions State KS KS KS KS KS KS KS KS KS KS KS KS KS KS KS KS KS KS KS KS KS KS KS KS KS KS KS KS KS KS KS KS KS KS KS KS KS KS KS KS KS KS KS Week Tested Positive % Pos 8/11/2013 8/18/2013 8/25/2013 9/1/2013 9/8/2013 9/15/2013 9/22/2013 9/29/2013 10/6/2013 10/13/2013 10/20/2013 10/27/2013 11/3/2013 11/10/2013 11/17/2013 11/24/2013 12/1/2013 12/8/2013 12/15/2013 12/22/2013 12/29/2013 1/5/2014 1/12/2014 1/19/2014 1/26/2014 2/2/2014 2/9/2014 2/16/2014 2/23/2014 3/2/2014 3/9/2014 3/16/2014 3/23/2014 3/30/2014 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 5/11/2014 5/18/2014 5/25/2014 6/1/2014 13 11 5 10 10 8 6 6 7 9 9 6 10 2 9 3 11 12 12 9 8 22 13 15 10 10 32 14 6 18 21 25 13 30 10 16 25 16 11 13 12 11 12 13 5 3 8 7 3 5

2 6 4 9 3 6 1 5 1 4 5 3 5 4 9 7 10 3 3 7 7 1 6 13 5 5 9 7 6 13 100.00% 45.45% 60.00% 80.00% 70.00% 37.50% 83.33% 33.33% 85.71% 44.44% 100.00% 50.00% 60.00% 50.00% 55.56% 33.33% 36.36% 41.67% 25.00% 55.56% 50.00% 40.91% 53.85% 66.67% 30.00% 30.00% 21.88% 50.00% 16.67% 33.33% 61.90% 20.00% 38.46% 30.00% 70.00% 37.50% 52.00% 0.00% 27.27% 30.77% 8.33% 27.27% 16.67% United States Department of Agriculture 3 4 1 3 2 Biological Samples Tested Positive 90 26 33 29 41 29 43 31 24 49 37 15 43 15 38 20 41 34 60 56 38 93 37 80 39 124 175 67 32 121 109 75 37 122 44 84 198 108 58 62 45 88 91 Environmental Samples Tested 89 12 14 20 25 16 20 7 22 27 35 6 27 14 19 1 8 13 8 19 14 28 12 40 7 17 32 25 2 30 23 22 10 55 36 16 81 3 16 1 44 5 Animal and Plant Health Inspection Service Positive 48 42 33 44 12 24 106 34 78 68 56 5 22 6 61 28 10 27 4 36 10 14 33 112 100 44 40 102 91 20 63 9 47 36 24 11 44 20 25 3 1 5 4 1 11 5 45 6 22 1 3 18 15 2 14 14 7 6 9 23 16 13 11 14 15 28 4 15 7 4 3 14 4

Veterinary Services 30 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Biological Accessions State KY KY KY KY KY KY KY KY KY KY KY KY KY KY KY KY KY KY KY KY KY KY KY KY KY KY KY KY KY KY KY KY MD MD MD MD MD MD MD MD Week Tested Positive % Pos TOTAL 7/28/2013 8/11/2013 10/6/2013 10/13/2013 10/20/2013 11/24/2013 12/8/2013 12/15/2013 12/22/2013 12/29/2013 1/5/2014 1/12/2014 1/19/2014 1/26/2014 2/2/2014 2/9/2014 2/16/2014 2/23/2014 3/2/2014 3/9/2014 3/16/2014 3/23/2014 3/30/2014 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 5/11/2014 5/18/2014 5/25/2014 6/1/2014 TOTAL 10/27/2013 12/29/2013 3/9/2014 3/23/2014 3/30/2014 4/6/2014 4/20/2014 6/1/2014 TOTAL 594 261 43.94% 0.00% 0.00% 100.00% 100.00% 100.00% 100.00% 0.00% 0.00% 0.00% 0.00% 16.67% 33.33% 14.29% 14.29% 16.67% 16.67% 0.00% 33.33% 0.00% 0.00% 20.00% 0.00% 25.00% 0.00% 0.00% 0.00% 0.00% 0.00% 16.67% 25.00% 0.00% 50.00% 14.52% 100.00% 0.00%

0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 10.00% 1 1 1 1 1 2 1 4 3 6 9 7 7 6 6 4 3 3 3 5 4 4 4 5 5 3 9 6 4 4 2 124 1 1 1 1 1 1 3 1 10 1 1 1 1 1 3 1 1 1 1 1 1 1 1 1 1 18 1 United States Department of Agriculture 1 Biological Samples Tested Positive 3127 2 1 2 1 2 8 4 15 4 14 21 12 12 16 22 10 5 9 7 18 11 21 12 37 17 7 51 43 19 9 8 420 1 1 2 3 3 3 6 1 20 Environmental Samples Tested Positive 1788 13 407 6 2 5 5 1151 1 2 1 2 8 9 1 2 6 2 1 6 8 1 1 8 3 54 1 32 7 1 0 0 Animal and Plant Health Inspection Service Veterinary Services 31 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Biological Accessions State ME MI MI MI MI MI MI MI MI MI MI MI MI MI MI MI MI MI MI MI MI MI MI MI MI MI MI MI MI MI MI MI MI MI MI MI MN MN MN MN MN Week Tested 10/27/2013 TOTAL 7/14/2013 7/28/2013 9/15/2013 10/6/2013 11/3/2013 11/10/2013 11/17/2013 11/24/2013 12/1/2013 12/8/2013 12/15/2013 12/22/2013

12/29/2013 1/5/2014 1/12/2014 1/19/2014 1/26/2014 2/2/2014 2/9/2014 2/16/2014 2/23/2014 3/2/2014 3/9/2014 3/16/2014 3/23/2014 3/30/2014 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 5/11/2014 5/18/2014 5/25/2014 6/1/2014 TOTAL 6/16/2013 6/23/2013 6/30/2013 7/7/2013 7/14/2013 1 1 1 1 1 1 3 3 2 1 7 9 3 5 4 6 10 10 6 14 21 11 14 7 23 20 14 26 20 15 17 17 15 20 14 15 11 367 25 47 28 21 32 Positive 0 1 2 2 2 2 1 4 4 2 4 9 7 10 3 13 16 9 17 10 9 6 11 9 10 3 6 5 177 8 4 United States Department of Agriculture 2 3 % Pos 0.00% 0.00% 100.00% 0.00% 0.00% 0.00% 66.67% 66.67% 0.00% 0.00% 0.00% 22.22% 0.00% 40.00% 0.00% 16.67% 40.00% 40.00% 33.33% 28.57% 42.86% 63.64% 71.43% 42.86% 56.52% 80.00% 64.29% 65.38% 50.00% 60.00% 35.29% 64.71% 60.00% 50.00% 21.43% 40.00% 45.45% 48.23% 32.00% 8.51% 0.00% 9.52% 9.38% Biological Samples Tested Positive 1 1 1 5 1 1 4 8 5 1 35 29 15 16 10 8 23 39 10 41 62 39 102 20 72 155 135 182 154 154 145 96 151 193 484 150 147 2693 87 173 63 48 67

Environmental Samples Tested Positive 0 0 5 1 10 1 3 16 3 33 21 50 11 28 30 22 24 22 8 284 2 8 2 14 14 11 4 4 2 6 4 2 14 14 10 4 0 1 3 7 3 8 2 11 13 5 4 24 35 37 3 33 98 77 71 82 65 65 36 76 56 15 35 53 918 20 7 3 7 Animal and Plant Health Inspection Service 75 Veterinary Services 32 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Biological Accessions State MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN Week Tested 7/21/2013 7/28/2013 8/4/2013 8/11/2013 8/18/2013 8/25/2013 9/1/2013 9/8/2013 9/15/2013 9/22/2013 9/29/2013 10/6/2013 10/13/2013 10/20/2013 10/27/2013 11/3/2013 11/10/2013 11/17/2013 11/24/2013 12/1/2013 12/8/2013 12/15/2013 12/22/2013 12/29/2013 1/5/2014 1/12/2014 1/19/2014 1/26/2014 2/2/2014 2/9/2014 2/16/2014 2/23/2014 3/2/2014 3/9/2014 3/16/2014 3/23/2014 3/30/2014 4/6/2014 4/13/2014 4/20/2014

4/27/2014 5/4/2014 5/11/2014 33 42 63 62 50 37 34 49 53 41 45 55 46 44 59 76 65 77 80 92 118 115 84 74 160 129 235 176 229 218 184 223 195 218 167 212 217 200 218 187 188 190 195 Positive 3 1 2 1 2 1 2 1 6 2 5 7 8 17 19 23 18 27 17 20 46 31 51 50 76 62 59 61 47 52 40 45 48 49 46 36 40 33 35 United States Department of Agriculture % Pos 0.00% 7.14% 1.59% 0.00% 0.00% 5.41% 2.94% 0.00% 3.77% 2.44% 4.44% 1.82% 13.04% 4.55% 8.47% 9.21% 12.31% 22.08% 23.75% 25.00% 15.25% 23.48% 20.24% 27.03% 28.75% 24.03% 21.70% 28.41% 33.19% 28.44% 32.07% 27.35% 24.10% 23.85% 23.95% 21.23% 22.12% 24.50% 21.10% 19.25% 21.28% 17.37% 17.95% Biological Samples Tested Positive 79 144 162 208 129 116 65 105 223 112 82 151 124 129 127 178 169 172 193 234 246 251 244 185 417 300 428 483 573 591 519 553 522 654 521 622 1107 740 725 672 698 640 627 Environmental Samples Tested Positive 22 14 53 27 17 28 8 28 4 38 70 121 91 52 51 47 112 109 87 200 92 103 16 21 103 341 134 212 126 110 109 133 137 77 98 180

145 101 179 63 71 68 62 8 7 24 9 1 2 2 9 5 14 5 25 27 9 18 37 29 63 58 33 51 84 50 87 68 105 84 129 126 123 112 103 145 93 80 238 91 78 67 88 68 96 Animal and Plant Health Inspection Service 1 46 55 23 8 2 19 34 41 97 27 13 1 7 36 108 43 40 71 26 32 55 34 11 14 59 48 18 27 5 12 17 1 Veterinary Services 33 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Biological Accessions State MN MN MN MO MO MO MO MO MO MO MO MO MO MO MO MO MO MO MO MO MO MO MO MO MO MO MO MO MO MO MO MO MO MO MO MO MO MO MO MO MO MO Week Tested Positive % Pos 5/18/2014 5/25/2014 6/1/2014 TOTAL 6/16/2013 6/23/2013 6/30/2013 7/7/2013 7/14/2013 7/21/2013 7/28/2013 8/4/2013 8/11/2013 8/18/2013 8/25/2013 9/1/2013 9/8/2013 9/15/2013 9/22/2013 9/29/2013 10/6/2013 10/13/2013 10/20/2013 10/27/2013 11/3/2013 11/10/2013 11/17/2013 11/24/2013 12/1/2013 12/8/2013 12/15/2013 12/22/2013 12/29/2013 1/5/2014 1/12/2014 1/19/2014 1/26/2014

2/2/2014 2/9/2014 2/16/2014 2/23/2014 3/2/2014 3/9/2014 176 165 93 5822 8 4 3 3 7 5 4 6 6 5 5 5 7 4 4 6 5 5 2 6 4 5 6 3 9 17 13 5 7 11 11 16 13 21 23 26 28 27 28 27 23 16 1172 15.34% 13.94% 17.20% 20.13% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 50.00% 20.00% 0.00% 0.00% 0.00% 17.65% 38.46% 40.00% 57.14% 0.00% 36.36% 43.75% 53.85% 42.86% 17.39% 15.38% 28.57% 40.74% 25.00% 2 1 3 5 2 4 4 7 7 9 4 4 8 11 7 United States Department of Agriculture Biological Samples Tested Positive 616 583 291 17148 20 8 4 5 10 8 4 11 9 7 7 12 12 4 4 10 10 8 2 15 5 8 9 16 79 60 97 74 23 35 27 59 52 124 73 74 97 96 101 Environmental Samples Tested Positive 112 37 42 4081 16 2 4 1106 62 77 37 2725 8 10 3 4 29 37 15 18 9 31 31 39 15 8 40 31 24 Animal and Plant Health Inspection Service 1 1 7 7 6 8 56 6 1 6 16 32 10 8 66 14 8 37 2 17 18 1 6 8 9 9 5 6 6 Veterinary Services 34 Source:

http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Biological Accessions State MO MO MO MO MO MO MO MO MO MO MO MO MS MT MT MT MT MT MT MT MT MT MT NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC Week Tested Positive % Pos 3/16/2014 3/23/2014 3/30/2014 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 5/11/2014 5/18/2014 5/25/2014 6/1/2014 TOTAL 3/30/2014 TOTAL 7/28/2013 1/12/2014 2/2/2014 3/2/2014 3/9/2014 3/23/2014 3/30/2014 4/6/2014 4/13/2014 5/4/2014 TOTAL 6/16/2013 6/23/2013 6/30/2013 7/7/2013 7/14/2013 7/21/2013 7/28/2013 8/4/2013 8/11/2013 8/18/2013 8/25/2013 9/1/2013 9/8/2013 9/15/2013 9/22/2013 9/29/2013 10/6/2013 34 34 20 30 28 21 17 43 47 24 41 39 751 1 1 1 1 1 17 16 8 12 7 9 5 17 14 5 8 9 205 1 1 50.00% 47.06% 40.00% 40.00% 25.00% 42.86% 29.41% 39.53% 29.79% 20.83% 19.51% 23.08% 27.30% 100.00% 100.00% 0.00% 0.00% 100.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 11.11% 0.00% 28.57% 66.67% 37.50%

40.00% 53.85% 16.67% 66.67% 62.50% 50.00% 66.67% 25.00% 30.00% 60.00% 82.76% 79.17% 58.33% 1 1 1 1 1 1 9 1 7 3 8 5 13 12 3 8 2 6 16 20 20 29 24 36 1 1 2 2 3 2 7 2 2 5 1 4 4 6 12 24 19 21 United States Department of Agriculture Biological Samples Tested Positive 116 165 191 203 134 56 66 203 389 79 464 370 3715 1 1 3 1 3 26 1 2 1 1 1 39 1 29 19 23 37 69 24 10 29 4 76 70 113 146 180 112 209 Environmental Samples Tested 62 40 47 32 32 24 17 45 67 17 41 70 828 1 1 1 13 29 7 4 68 73 43 84 263 56 67 999 Positive 4 11 1 20 17 6 55 27 245 3 1 2 3 4 3 16 18 14 30 42 5 9 12 1 21 12 11 61 114 90 123 Animal and Plant Health Inspection Service 10 0 8 2 1 1 5 6 2 1 5 6 2 Veterinary Services 35 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Biological Accessions State NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC ND ND ND ND ND ND ND ND Week

Tested Positive % Pos 10/13/2013 10/20/2013 10/27/2013 11/3/2013 11/10/2013 11/17/2013 11/24/2013 12/1/2013 12/8/2013 12/15/2013 12/22/2013 12/29/2013 1/5/2014 1/12/2014 1/19/2014 1/26/2014 2/2/2014 2/9/2014 2/16/2014 2/23/2014 3/2/2014 3/9/2014 3/16/2014 3/23/2014 3/30/2014 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 5/11/2014 5/18/2014 5/25/2014 6/1/2014 TOTAL 12/8/2013 12/15/2013 1/5/2014 1/19/2014 2/2/2014 2/9/2014 2/16/2014 2/23/2014 21 22 17 15 15 13 41 56 46 46 25 48 55 23 72 17 90 71 98 98 73 66 82 82 92 92 87 49 86 88 92 123 89 60 2263 21 22 17 14 15 12 19 8 12 19 5 17 15 20 19 15 24 23 24 23 26 16 17 20 24 23 20 9 16 21 18 23 15 13 721 100.00% 100.00% 100.00% 93.33% 100.00% 92.31% 46.34% 14.29% 26.09% 41.30% 20.00% 35.42% 27.27% 86.96% 26.39% 88.24% 26.67% 32.39% 24.49% 23.47% 35.62% 24.24% 20.73% 24.39% 26.09% 25.00% 22.99% 18.37% 18.60% 23.86% 19.57% 18.70% 16.85% 21.67% 31.86% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 33.33% 1 1 2 3 3 3 United States Department

of Agriculture 1 Biological Samples Tested Positive 91 109 127 50 120 52 482 547 773 433 323 616 589 311 922 223 627 931 1206 947 613 415 683 729 775 719 879 758 1129 1107 1529 1645 1210 852 23673 Environmental Samples Tested Positive 4 1 4 1 15 21 3 13 27 31 2 25 23 2 48 2 525 80 59 65 86 214 187 134 124 374 342 340 253 115 191 141 54 97 3622 5 14 16 2 8 7 19 1 12 16 2 13 2 136 27 9 17 17 95 77 50 96 85 66 92 91 25 18 35 5 23 1096 91 109 127 48 120 51 164 47 171 123 66 150 159 147 238 137 127 226 300 200 105 54 91 126 112 113 100 55 115 228 95 205 124 100 5003 3 1 10 8 11 9 3 6 4 11 5 1 Animal and Plant Health Inspection Service Veterinary Services 36 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Biological Accessions State ND ND ND ND ND ND ND ND ND ND ND ND ND NE NE NE NE NE NE NE NE NE NE NE NE NE NE NE NE NE NE NE NE NE NE NE NE NE NE NE NE NE Week Tested 3/9/2014 3/16/2014 3/23/2014

3/30/2014 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 5/11/2014 5/18/2014 5/25/2014 6/1/2014 TOTAL 6/23/2013 6/30/2013 7/14/2013 7/21/2013 7/28/2013 8/11/2013 8/18/2013 8/25/2013 9/15/2013 9/29/2013 10/6/2013 10/13/2013 10/27/2013 11/3/2013 11/10/2013 11/17/2013 11/24/2013 12/1/2013 12/8/2013 12/15/2013 12/22/2013 12/29/2013 1/5/2014 1/12/2014 1/19/2014 1/26/2014 2/2/2014 2/9/2014 2/16/2014 3 5 1 3 Positive 2 2 2 3 4 1 39 1 1 3 2 3 2 2 3 1 1 3 3 2 3 3 6 10 14 10 21 21 29 25 35 32 51 30 39 United States Department of Agriculture 1 1 1 1 2 1 5 2 7 4 9 6 % Pos 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 2.56% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 10.00% 0.00% 10.00% 4.76% 9.52% 3.45% 20.00% 5.71% 21.88% 7.84% 30.00% 15.38% Biological Samples Tested Positive Environmental Samples Tested 6 20 1 16 15 26 9 16 19 15 16 8 4 2 4 10 7 3 115 1 3 11 3 7 2 5 31 1 3 5 6 8 5 17 16 25 32

29 60 60 72 78 126 138 271 178 207 Positive 1 30 25 213 14 3 4 4 1 1 12 8 5 4 3 3 29 44 3 1 1 2 1 15 3 46 7 68 51 Animal and Plant Health Inspection Service 25 39 26 36 10 17 6 32 23 49 4 30 16 13 2 2 4 1 2 3 Veterinary Services 37 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Biological Accessions State NE NE NE NE NE NE NE NE NE NE NE NE NE NE NE NH NH NY NY NY NY NY NY NY NY NY OH OH OH OH OH OH OH OH OH OH OH OH OH OH Week Tested Positive % Pos 2/23/2014 3/2/2014 3/9/2014 3/16/2014 3/23/2014 3/30/2014 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 5/11/2014 5/18/2014 5/25/2014 6/1/2014 TOTAL 4/6/2014 4/13/2014 TOTAL 6/16/2013 9/15/2013 12/15/2013 12/22/2013 2/9/2014 3/9/2014 3/16/2014 5/25/2014 6/1/2014 TOTAL 6/16/2013 6/30/2013 7/7/2013 7/14/2013 7/21/2013 7/28/2013 8/4/2013 8/11/2013 8/18/2013 8/25/2013 9/1/2013 9/8/2013 9/15/2013 9/22/2013 46 29 41 34 35 33 42 39 41 33 38 30 23 40

30 890 1 1 2 1 1 1 1 1 2 1 1 1 10 5 4 3 2 3 3 4 2 2 2 12 2 5 6 9 7 10 2 12 7 6 4 9 10 4 144 1 1 6 1 26.09% 6.90% 12.20% 17.65% 25.71% 21.21% 23.81% 5.13% 29.27% 21.21% 15.79% 13.33% 39.13% 25.00% 13.33% 16.18% 0.00% 0.00% 0.00% 100.00% 100.00% 0.00% 0.00% 100.00% 50.00% 100.00% 0.00% 0.00% 50.00% 60.00% 50.00% 66.67% 0.00% 0.00% 33.33% 0.00% 0.00% 100.00% 50.00% #DIV/0! 100.00% 0.00% 0.00% United States Department of Agriculture 0 1 1 1 1 1 5 3 2 2 1 2 1 Biological Samples Tested Positive 285 98 141 143 189 186 297 194 369 254 265 253 222 378 359 5033 9 2 11 1 3 2 1 1 12 5 1 1 27 18 15 28 2 9 12 8 2 3 5 75 7 13 28 35 44 43 8 134 93 28 38 45 113 95 997 16 1 6 16 Environmental Samples Tested Positive 27 5 24 9 30 9 46 42 9 14 11 20 109 44 6 859 6 139 0 0 0 1 3 3 31 30 6 2 42 2 1 2 5 12 12 13 24 2 1 5 3 2 Animal and Plant Health Inspection Service 2 4 8 17 28 12 13 0 2 1 3 Veterinary Services 38 Source: http://www.doksinet SECD Testing Summary

Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Biological Accessions State OH OH OH OH OH OH OH OH OH OH OH OH OH OH OH OH OH OH OH OH OH OH OH OH OH OH OH OH OH OH OH OH OH OH OH OH OK OK OK OK OK OK Week Tested 9/29/2013 10/6/2013 10/13/2013 10/20/2013 10/27/2013 11/3/2013 11/10/2013 11/17/2013 11/24/2013 12/1/2013 12/8/2013 12/15/2013 12/22/2013 12/29/2013 1/5/2014 1/12/2014 1/19/2014 1/26/2014 2/2/2014 2/9/2014 2/16/2014 2/23/2014 3/2/2014 3/9/2014 3/16/2014 3/23/2014 3/30/2014 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 5/11/2014 5/18/2014 5/25/2014 6/1/2014 TOTAL 6/16/2013 6/23/2013 6/30/2013 7/7/2013 7/14/2013 7/21/2013 4 4 6 7 7 16 20 17 19 39 37 33 24 35 35 34 55 51 57 62 61 48 50 42 63 56 54 66 34 41 45 45 39 47 21 25 1337 24 24 12 17 14 14 Positive 1 5 4 4 9 5 3 3 5 5 7 9 10 11 11 21 22 19 19 13 17 16 17 22 10 9 12 7 7 3 1 319 16 13 3 11 7 4 United States Department of Agriculture % Pos 0.00% 0.00% 0.00% 0.00% 14.29% 31.25% 20.00%

23.53% 47.37% 12.82% 8.11% 9.09% 20.83% 14.29% 20.00% 26.47% 18.18% 21.57% 19.30% 33.87% 36.07% 39.58% 38.00% 30.95% 26.98% 28.57% 31.48% 33.33% 29.41% 21.95% 26.67% 15.56% 17.95% 6.38% 4.76% 0.00% 23.86% 66.67% 54.17% 25.00% 64.71% 50.00% 28.57% Biological Samples Tested Positive 16 20 16 31 13 26 67 69 99 174 168 108 81 100 118 148 215 203 402 241 208 160 232 146 190 213 203 314 153 171 377 321 284 263 143 136 5954 248 241 133 195 95 122 Environmental Samples Tested Positive 29 14 2 1 30 12 30 26 38 38 51 23 16 22 15 10 84 54 29 85 38 54 42 20 24 33 33 33 45 13 29 19 20 21 27 26 10 1182 98 169 105 47 82 44 2 5 3 5 2 5 11 5 1 9 12 5 56 9 6 11 11 13 14 23 27 42 64 75 55 56 69 31 32 38 52 82 16 28 65 47 31 15 1 1067 75 104 23 61 36 10 Animal and Plant Health Inspection Service 12 17 10 13 8 18 2 1 1 4 3 1 10 1 2 3 153 4 72 22 10 16 16 Veterinary Services 39 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June

4, 2014 Biological Accessions State OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK Week Tested Positive % Pos 7/28/2013 8/4/2013 8/11/2013 8/18/2013 8/25/2013 9/1/2013 9/8/2013 9/15/2013 9/22/2013 9/29/2013 10/6/2013 10/13/2013 10/20/2013 10/27/2013 11/3/2013 11/10/2013 11/17/2013 11/24/2013 12/1/2013 12/8/2013 12/15/2013 12/22/2013 12/29/2013 1/5/2014 1/12/2014 1/19/2014 1/26/2014 2/2/2014 2/9/2014 2/16/2014 2/23/2014 3/2/2014 3/9/2014 3/16/2014 3/23/2014 3/30/2014 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 5/11/2014 5/18/2014 15 14 16 23 18 14 21 12 13 14 11 19 13 20 13 9 16 36 30 32 12 12 3 16 17 20 16 17 23 32 24 21 28 31 17 27 31 33 31 36 45 47 35 10 5 7 14 10 4 13 5 4 6 6 13 9 13 10 5 11 12 6 7 5 3 66.67% 35.71% 43.75% 60.87% 55.56% 28.57% 61.90% 41.67% 30.77% 42.86% 54.55% 68.42% 69.23% 65.00% 76.92% 55.56% 68.75% 33.33% 20.00% 21.88% 41.67% 25.00% 0.00% 25.00% 47.06% 10.00% 25.00%

23.53% 26.09% 28.13% 20.83% 4.76% 32.14% 29.03% 29.41% 18.52% 32.26% 12.12% 29.03% 16.67% 22.22% 17.02% 11.43% 4 8 2 4 4 6 9 5 1 9 9 5 5 10 4 9 6 10 8 4 United States Department of Agriculture Biological Samples Tested Positive 86 93 98 132 101 109 101 95 71 63 44 82 70 101 88 36 107 255 240 159 87 90 14 145 83 76 79 239 224 248 481 204 156 166 104 237 182 248 243 254 405 381 325 Environmental Samples Tested Positive 18 4 16 79 16 3 8 92 8 105 12 11 50 151 60 93 77 47 12 29 91 29 9 38 69 51 56 147 60 88 73 122 82 65 111 133 249 36 73 135 7 208 101 6 1 22 10 39 34 22 6 26 30 4 18 31 63 49 65 60 17 56 48 30 25 30 9 8 18 16 24 77 68 55 45 15 50 49 12 12 43 20 20 8 74 94 9 Animal and Plant Health Inspection Service 2 2 3 3 1 6 7 12 6 46 2 5 5 9 4 1 3 15 13 25 27 6 38 10 34 11 8 2 17 140 2 3 19 78 1 Veterinary Services 40 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Biological Accessions State OK

OK OR OR OR OR PA PA PA PA PA PA PA PA PA PA PA PA PA PA PA PA PA PA PA PA PA PA PA PA PA PA PA PA PA PA PA PA PA PA PA Week Tested Positive % Pos 5/25/2014 6/1/2014 TOTAL 3/30/2014 4/20/2014 4/27/2014 5/18/2014 TOTAL 8/25/2013 9/1/2013 9/15/2013 9/29/2013 10/6/2013 10/13/2013 10/20/2013 10/27/2013 11/3/2013 11/10/2013 11/17/2013 11/24/2013 12/1/2013 12/8/2013 12/15/2013 12/22/2013 12/29/2013 1/5/2014 1/12/2014 1/19/2014 1/26/2014 2/2/2014 2/9/2014 2/16/2014 2/23/2014 3/2/2014 3/9/2014 3/16/2014 3/23/2014 3/30/2014 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 27 36 1101 1 1 3 1 6 3 5 1 1 1 2 1 2 1 1 1 1 9 7 13 1 2 11 2 15 4 13 13 17 34 14 19 10 8 9 10 8 13 1 11 4 4 362 14.81% 11.11% 32.88% 0.00% 0.00% 0.00% 0.00% 0.00% 100.00% 100.00% 100.00% 100.00% 100.00% 100.00% 100.00% 100.00% 100.00% 100.00% 100.00% 0.00% 33.33% 14.29% 7.69% 0.00% 0.00% 0.00% 100.00% 13.33% 100.00% 30.77% 23.08% 41.18% 35.29% 21.43% 15.79% 50.00% 12.50% 11.11% 20.00% 25.00% 23.08% 0.00% 9.09% 0 3 5 1

1 1 2 1 2 1 1 1 3 1 1 2 2 4 4 3 7 12 3 3 5 1 1 2 2 3 United States Department of Agriculture 1 Biological Samples Tested Positive 306 305 8447 1 1 8 6 16 6 9 1 2 1 28 1 2 1 21 5 1 24 18 41 2 13 17 9 117 11 32 16 27 79 47 68 14 27 24 26 75 17 2 64 Environmental Samples Tested Positive 73 12 1805 52 99 3620 6 5 724 0 6 9 1 2 1 28 1 2 1 21 5 0 0 1 1 2 2 23 5 4 1 2 3 1 4 6 2 10 7 6 9 14 10 3 7 1 2 2 2 3 4 30 30 29 1 50 13 1 1 Animal and Plant Health Inspection Service Veterinary Services 41 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Biological Accessions State PA PA PA PA SC SC SC SC SC SC SC SC SC SC SC SC SD SD SD SD SD SD SD SD SD SD SD SD SD SD SD SD SD SD SD SD SD SD SD SD SD Week Tested Positive % Pos 5/11/2014 5/18/2014 5/25/2014 6/1/2014 TOTAL 8/11/2013 1/5/2014 1/12/2014 1/19/2014 2/2/2014 2/9/2014 2/16/2014 2/23/2014 3/2/2014 4/6/2014 4/13/2014 4/27/2014 TOTAL

6/16/2013 6/23/2013 6/30/2013 7/7/2013 7/14/2013 7/21/2013 7/28/2013 8/4/2013 8/11/2013 8/18/2013 8/25/2013 9/1/2013 9/8/2013 9/15/2013 9/22/2013 9/29/2013 10/6/2013 10/13/2013 10/20/2013 10/27/2013 11/3/2013 11/10/2013 11/17/2013 11/24/2013 12/1/2013 24 8 12 3 311 1 2 1 2 1 1 5 1 3 1 2 12.50% 12.50% 16.67% 0.00% 27.33% 0.00% 100.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 9.52% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 9.09% 0.00% 85 1 3 3 21 2 5 3 3 2 1 7 6 1 5 7 4 6 10 11 5 6 9 5 13 9 13 12 11 9 United States Department of Agriculture 2 2 1 Biological Samples Tested Positive 141 52 36 3 1080 1 39 10 10 2 3 22 3 2 10 7 109 2 19 4 3 3 5 13 20 3 9 11 5 11 24 27 12 12 23 10 32 41 31 29 30 29 Environmental Samples Tested 14 1 4 189 1 52 1 60 259 Positive 1 55 3 6 3 1 1 4 3 11 6 4 9 1 1 4 5 1 Animal and Plant Health Inspection Service 5 1

Veterinary Services 42 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Biological Accessions State SD SD SD SD SD SD SD SD SD SD SD SD SD SD SD SD SD SD SD SD SD SD SD SD SD SD TN TN TN TN TN TN TN TN TN TN TN TN TN TN TN TN Week Tested 12/8/2013 12/15/2013 12/22/2013 12/29/2013 1/5/2014 1/12/2014 1/19/2014 1/26/2014 2/2/2014 2/9/2014 2/16/2014 2/23/2014 3/2/2014 3/9/2014 3/16/2014 3/23/2014 3/30/2014 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 5/11/2014 5/18/2014 5/25/2014 6/1/2014 TOTAL 6/16/2013 7/7/2013 7/21/2013 8/4/2013 8/11/2013 8/18/2013 8/25/2013 9/8/2013 9/29/2013 10/20/2013 10/27/2013 11/3/2013 11/17/2013 11/24/2013 12/1/2013 12/22/2013 11 16 11 11 26 24 25 26 30 28 30 32 42 37 35 31 41 33 35 37 40 39 38 42 43 18 946 1 1 1 Positive 2 1 2 3 3 3 4 5 3 6 7 6 5 5 2 4 3 5 5 4 6 4 3 92 1 1 1 1 1 1 2 1 2 United States Department of Agriculture 1 1 1 1 1 % Pos 0.00% 0.00% 0.00% 18.18%

3.85% 8.33% 12.00% 11.54% 10.00% 14.29% 16.67% 9.38% 14.29% 18.92% 17.14% 16.13% 12.20% 6.06% 11.43% 8.11% 12.50% 12.82% 10.53% 14.29% 9.30% 16.67% 9.73% 0.00% 0.00% 100.00% 0.00% 0.00% 0.00% 0.00% 100.00% 0.00% 0.00% 100.00% 100.00% 100.00% 0.00% 0.00% 0.00% Biological Samples Tested Positive 36 43 17 35 104 64 67 55 92 52 64 99 155 150 154 91 149 152 205 194 183 164 149 167 220 56 3325 2 4 5 3 1 Environmental Samples Tested Positive 1 5 5 2 2 9 4 4 5 6 10 18 16 20 7 21 4 5 12 11 8 8 20 10 8 216 10 7 23 17 21 2 4 2 1 3 6 1 30 11 1 1 6 7 1 8 10 3 1 8 4 6 3 4 1 202 38 5 10 15 7 10 1 13 4 5 2 4 10 24 13 5 2 4 Animal and Plant Health Inspection Service 3 25 3 5 5 Veterinary Services 43 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Biological Accessions State TN TN TN TN TN TN TN TN TN TN TN TN TN TN TN TN TN TN TN TN TN TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX

Week Tested Positive % Pos 12/29/2013 1/5/2014 1/12/2014 1/19/2014 1/26/2014 2/2/2014 2/9/2014 2/16/2014 2/23/2014 3/2/2014 3/9/2014 3/16/2014 3/23/2014 3/30/2014 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 5/11/2014 5/25/2014 TOTAL 6/16/2013 6/23/2013 7/7/2013 7/14/2013 7/21/2013 7/28/2013 8/4/2013 8/11/2013 8/25/2013 9/29/2013 10/6/2013 10/13/2013 10/20/2013 10/27/2013 11/3/2013 11/10/2013 11/17/2013 11/24/2013 12/1/2013 12/8/2013 12/15/2013 3 4 6 3 1 33.33% 0.00% 0.00% 33.33% 0.00% 0.00% 0.00% 25.00% 100.00% 0.00% 0.00% 0.00% 0.00% 0.00% 40.00% 0.00% 33.33% 50.00% 0.00% 0.00% 0.00% 20.31% 0.00% 0.00% 0.00% 0.00% 50.00% 0.00% 0.00% 0.00% 0.00% 100.00% 0.00% 0.00% 0.00% 100.00% 33.33% 100.00% 66.67% 42.86% 100.00% 50.00% 0.00% 1 4 1 1 6 1 3 1 5 1 3 2 1 1 3 64 2 4 1 4 2 1 1 3 3 3 3 1 1 3 3 3 9 7 2 2 1 1 1 1 2 1 1 13 United States Department of Agriculture 1 3 3 1 3 6 3 2 1 Biological Samples Tested Positive 17 18 41 15 2 19 3 3 53 1 9 3 47 3 8 8 8 12 9 356 8 14 4

12 6 1 6 10 7 11 4 5 1 9 11 13 66 35 6 8 5 Environmental Samples Tested Positive 10 10 22 15 2 3 3 31 3 4 2 1 7 8 3 3 7 1 3 7 49 180 29 2 2 10 7 3 10 21 13 4 4 Animal and Plant Health Inspection Service 17 17 Veterinary Services 44 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Biological Accessions State TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX UT UT UT UT UT VA VA VA VA VA VA VA VA VA VA VA VA Week Tested Positive % Pos 12/22/2013 12/29/2013 1/5/2014 1/12/2014 1/19/2014 1/26/2014 2/2/2014 2/9/2014 2/16/2014 2/23/2014 3/2/2014 3/9/2014 3/16/2014 3/23/2014 3/30/2014 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 5/11/2014 5/18/2014 5/25/2014 6/1/2014 TOTAL 12/8/2013 3/16/2014 3/23/2014 4/6/2014 4/20/2014 TOTAL 9/1/2013 10/27/2013 12/1/2013 12/8/2013 1/12/2014 2/2/2014 2/9/2014 3/23/2014 4/6/2014 4/13/2014 4/27/2014 5/11/2014 2 1 6 3 2 2 3 3 4 5 6

10 4 5 4 5 5 10 8 14 6 1 3 1 172 1 1 2 1 4 3 1 2 1 2 2 2 5 6 2 1 2 3 3 5 2 4 3 100.00% 100.00% 66.67% 100.00% 50.00% 100.00% 33.33% 66.67% 50.00% 40.00% 83.33% 60.00% 50.00% 20.00% 50.00% 60.00% 60.00% 50.00% 25.00% 28.57% 50.00% 0.00% 33.33% 0.00% 46.51% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 100.00% 0.00% 0.00% 1 80 1 2 5 0 1 2 4 1 1 1 1 1 1 2 United States Department of Agriculture 1 Biological Samples Tested Positive 10 6 20 18 11 12 15 11 20 23 27 23 30 15 12 13 35 53 30 232 28 6 15 2 909 3 1 Environmental Samples Tested Positive 12 11 13 12 10 6 10 9 9 4 6 8 8 10 20 10 3 3 4 9 10 13 3 11 8 10 10 14 8 18 7 3 25 20 33 16 10 59 251 244 92 22 10 1 3 8 0 8 18 2 0 2 1 22 8 2 1 1 1 1 1 2 3 1 Animal and Plant Health Inspection Service 8 Veterinary Services 45 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Biological Accessions

State VA VA VT WI WI WI WI WI WI WI WI WI WI WI WI WI WI WI WI WI WI WI WI WI WI WI WI WI WI WI WI WI WI WI WI WI WV WV WY Week Tested 5/25/2014 6/1/2014 TOTAL 3/23/2014 TOTAL 7/7/2013 7/28/2013 8/4/2013 9/8/2013 10/27/2013 11/3/2013 11/24/2013 12/1/2013 12/15/2013 12/22/2013 12/29/2013 1/5/2014 1/12/2014 1/19/2014 1/26/2014 2/2/2014 2/9/2014 2/16/2014 2/23/2014 3/2/2014 3/9/2014 3/16/2014 3/23/2014 3/30/2014 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 5/11/2014 5/18/2014 5/25/2014 6/1/2014 TOTAL 4/20/2014 4/27/2014 TOTAL 12/29/2013 1 16 1 1 1 1 1 2 2 1 1 2 2 2 2 5 1 9 2 5 3 6 4 4 4 2 7 3 8 5 6 5 2 4 10 5 2 119 1 2 3 1 Positive 1 1 1 1 1 1 1 1 1 1 1 2 1 2 1 1 3 18 United States Department of Agriculture 3 1 % Pos 0.00% 0.00% 6.25% 100.00% 100.00% 0.00% 0.00% 100.00% 0.00% 50.00% 100.00% 100.00% 0.00% 0.00% 0.00% 0.00% 0.00% 100.00% 0.00% 50.00% 20.00% 33.33% 33.33% 0.00% 25.00% 0.00% 0.00% 28.57% 0.00% 0.00% 20.00% 0.00% 0.00% 0.00% 25.00% 30.00% 0.00% 0.00% 15.13%

0.00% 0.00% 100.00% 100.00% Biological Samples Tested Positive Environmental Samples Tested Positive 2 40 82 3 3 2 1 3 2 2 2 3 3 6 4 6 13 1 39 3 15 8 14 4 4 8 2 15 4 12 12 13 56 2 9 47 11 5 331 1 3 4 10 1 3 3 13 2 3 1 2 3 3 1 1 1 1 2 1 5 1 1 1 8 1 1 1 8 3 5 3 3 1 6 9 1 35 40 2 0 10 0 0 Animal and Plant Health Inspection Service Veterinary Services 46 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Biological Accessions Biological Samples Tested Positive State WY WY WY WY WY WY WY WY WY WY WY WY WY WY WY WY WY WY WY Week Tested 1/5/2014 1/19/2014 1/26/2014 2/2/2014 2/9/2014 2/16/2014 2/23/2014 3/2/2014 3/9/2014 3/16/2014 3/23/2014 3/30/2014 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 5/18/2014 5/25/2014 TOTAL 1 1 1 1 2 1 2 Positive % Pos 0.00% 0.00% 100.00% 100.00% 100.00% 100.00% 100.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 50.00% 0.00% 0.00% 0.00% 56.25% 1 1

2 1 2 1 2 2 1 1 16 9 1 1 6 10 15 10 25 Environmental Samples Tested Positive 29 10 10 10 28 25 25 26 25 25 5 6 1 10 10 15 1 26 2 1 1 108 53 9 4 4 5 4 4 3 25 231 45 Appendix B. Weekly Testing Data for PDCoV by State Biological Accessions State AR AR AR AR AZ CA CO CO CO CO CO CO CO Week Tested 4/13/2014 4/20/2014 4/27/2014 5/4/2014 Total 4/13/2014 Total 5/18/2014 Total 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 5/11/2014 5/18/2014 3 2 1 1 7 1 1 1 1 1 Positive 0 0 0 % Pos 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% Biological Samples Tested Positive 27 3 1 1 32 1 1 1 0 5 Environmental Samples Tested Positive 0 0 0 0 0 0 0 0 0 23 3 10 1 1 3 United States Department of Agriculture 0.00% 0.00% 0.00% 0.00% 0.00% 8 48 6 4 18 Animal and Plant Health Inspection Service 5 9 Veterinary Services 47 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014

Biological Accessions State CO GA IA IA IA IA IA IA IA IA IA IA ID IL IL IL IL IL IL IL IL IL IL IN IN IN IN IN IN IN IN IN IN IN KS KS KS Week Tested 5/25/2014 Total 5/18/2014 Total 3/30/2014 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 5/11/2014 5/18/2014 5/25/2014 6/1/2014 Total 5/4/2014 Total 3/30/2014 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 5/11/2014 5/18/2014 5/25/2014 6/1/2014 Total 3/23/2014 3/30/2014 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 5/11/2014 5/18/2014 5/25/2014 6/1/2014 Total 3/30/2014 4/6/2014 4/13/2014 1 20 1 1 22 23 25 31 29 20 22 25 23 10 232 1 1 24 35 37 24 44 29 23 19 36 20 291 1 14 20 18 12 15 17 11 14 12 9 143 1 7 2 United States Department of Agriculture Positive 0 0 3 4 5 2 3 3 2 22 0 4 2 8 3 7 10 2 2 5 2 45 1 7 4 3 3 5 3 3 2 4 35 % Pos 0.00% 0.00% 0.00% 0.00% 13.64% 17.39% 20.00% 6.45% 10.34% 15.00% 9.09% 0.00% 0.00% 0.00% 9.48% 0.00% 0.00% 16.67% 5.71% 21.62% 12.50% 15.91% 34.48% 8.70% 10.53% 13.89% 10.00% 15.46% 0.00% 7.14%

35.00% 22.22% 25.00% 20.00% 29.41% 27.27% 21.43% 16.67% 44.44% 24.48% 0.00% 0.00% 0.00% Biological Samples Tested Positive 3 92 3 3 65 91 69 105 68 87 50 75 85 25 726 7 7 190 224 186 119 269 199 254 151 300 210 2102 1 42 87 67 51 63 65 55 74 77 62 644 4 22 6 Environmental Samples Tested Positive 0 37 0 0 6 8 10 3 5 7 9 0 18 26 16 24 2 1 8 5 0 4 48 100 10 0 36 6 35 7 15 27 9 6 10 6 157 0 6 34 5 34 61 18 29 13 28 0 1 228 10 2 28 16 15 12 18 15 11 13 15 145 Animal and Plant Health Inspection Service 3 3 3 5 1 1 5 9 2 1 5 5 6 28 3 27 8 Veterinary Services 6 48 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Biological Accessions State KS KS KS KS KS KS KS KY KY KY KY KY KY KY MD MD MD MI MI MI MI MI MI MI MI MI MI MN MN MN MN MN MN MN MN MN MN MO Week Tested 4/20/2014 4/27/2014 5/4/2014 5/11/2014 5/18/2014 5/25/2014 6/1/2014 Total 3/30/2014 4/20/2014 5/4/2014 5/11/2014 5/18/2014

5/25/2014 6/1/2014 Total 3/30/2014 4/6/2014 4/27/2014 Total 3/30/2014 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 5/11/2014 5/18/2014 5/25/2014 6/1/2014 Total 3/30/2014 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 5/11/2014 5/18/2014 5/25/2014 6/1/2014 Total 3/30/2014 4 2 1 5 1 1 3 27 1 2 5 1 1 1 2 13 1 1 1 3 5 7 6 1 6 3 11 4 5 4 52 44 59 56 48 63 61 54 65 58 32 540 9 United States Department of Agriculture Positive 1 1 2 0 0 4 2 1 1 2 1 11 6 5 7 10 11 6 8 5 4 2 64 % Pos 0.00% 50.00% 0.00% 0.00% 0.00% 0.00% 33.33% 7.41% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 80.00% 28.57% 16.67% 0.00% 16.67% 66.67% 0.00% 25.00% 0.00% 0.00% 21.15% 13.64% 8.47% 12.50% 20.83% 17.46% 9.84% 14.81% 7.69% 6.90% 6.25% 11.85% 0.00% Biological Samples Tested Positive 18 2 2 17 1 1 11 84 10 8 14 2 2 2 8 46 3 3 1 7 14 24 44 6 21 8 73 12 17 42 261 199 154 129 92 139 138 138 139 131 71 1330 50 Environmental Samples Tested Positive 5 6 12 2 4 3 2 3 12 76 0 0 0

0 0 12 7 1 0 0 4 14 5 2 9 14 3 2 1 30 13 18 12 13 24 8 17 14 12 3 134 Animal and Plant Health Inspection Service 2 45 10 9 37 38 23 29 17 14 6 183 Veterinary Services 1 2 3 1 1 4 2 8 49 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Biological Accessions State MO MO MO MO MO MO MO MO MO MT MT NC NC NC NC NC NC NC NC NC NC ND ND ND ND ND NE NE NE NE NE NE NE NE NE NE NY Week Tested 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 5/11/2014 5/18/2014 5/25/2014 6/1/2014 Total 3/30/2014 4/6/2014 Total 3/30/2014 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 5/11/2014 5/18/2014 5/25/2014 6/1/2014 Total 3/30/2014 4/20/2014 4/27/2014 5/18/2014 6/1/2014 Total 3/30/2014 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 5/11/2014 5/18/2014 5/25/2014 6/1/2014 Total 5/25/2014 Total 5 10 6 7 10 11 10 3 4 75 2 1 3 6 7 11 6 17 12 11 12 12 6 100 1 2 1 3 7 4 4 5 10 7 12 10 6 8 1 67 1 1 United States

Department of Agriculture Positive 1 1 1 3 1 1 1 1 1 4 1 1 9 0 1 1 2 2 2 8 0 % Pos 0.00% 0.00% 16.67% 14.29% 10.00% 0.00% 0.00% 0.00% 0.00% 4.00% 50.00% 0.00% 33.33% 0.00% 0.00% 9.09% 16.67% 5.88% 33.33% 0.00% 8.33% 8.33% 0.00% 9.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 25.00% 0.00% 10.00% 28.57% 16.67% 20.00% 0.00% 0.00% 0.00% 11.94% 0.00% 0.00% Biological Samples Tested Positive 17 64 16 20 37 75 36 12 15 342 3 1 4 21 33 28 21 176 114 67 85 50 20 615 1 2 5 10 18 9 9 32 179 90 68 82 32 25 7 533 1 1 Environmental Samples Tested Positive 6 1 1 6 55 11 30 42 30 8 2 174 4 2 4 2 4 4 2 9 11 17 5 6 1 1 1 51 13 28 41 58 30 48 34 2 254 14 0 4 25 29 0 2 10 1 3 19 5 8 2 30 23 36 8 5 10 10 6 6 98 0 0 0 0 Animal and Plant Health Inspection Service Veterinary Services 50 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Biological Accessions State OH OH OH OH OH OH OH OH OH OH OH

OK OK OK OK OK OK OK OK OK OK PA PA PA PA PA PA PA PA PA SD SD SD SD SD SD SD SD SD SD Week Tested 3/23/2014 3/30/2014 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 5/11/2014 5/18/2014 5/25/2014 6/1/2014 Total 3/30/2014 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 5/11/2014 5/18/2014 5/25/2014 6/1/2014 Total 3/30/2014 4/6/2014 4/13/2014 4/20/2014 5/4/2014 5/11/2014 5/18/2014 5/25/2014 6/1/2014 Total 3/30/2014 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 5/11/2014 5/18/2014 5/25/2014 6/1/2014 1 28 42 15 22 17 31 23 26 17 17 239 4 5 10 12 10 16 12 14 6 15 104 3 5 2 7 3 8 3 3 1 35 5 7 13 15 20 18 21 18 22 11 United States Department of Agriculture Positive 6 7 1 3 2 5 3 3 2 2 34 1 1 1 1 3 1 1 7 1 1 1 2 3 2 % Pos 0.00% 21.43% 16.67% 6.67% 13.64% 11.76% 16.13% 13.04% 11.54% 11.76% 11.76% 14.23% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 6.67% 0.96% 0.00% 20.00% 50.00% 42.86% 33.33% 0.00% 0.00% 33.33% 0.00% 20.00% 0.00% 14.29% 0.00% 0.00% 5.00% 5.56% 9.52%

16.67% 0.00% 18.18% Biological Samples Tested Positive 4 97 197 43 64 89 309 147 150 102 55 1257 59 25 77 58 76 175 65 103 21 54 713 7 12 2 14 3 31 7 4 1 81 30 27 38 52 50 65 65 51 86 30 21 23 1 7 5 20 6 4 3 5 95 Environmental Samples Tested Positive 25 27 13 17 17 12 13 17 22 8 171 24 125 6 24 9 6 27 53 11 10 295 12 10 2 1 2 10 3 2 1 8 9 2 4 3 2 3 8 1 1 1 1 6 1 22 0 1 4 2 3 4 Animal and Plant Health Inspection Service Veterinary Services 51 Source: http://www.doksinet SECD Testing Summary Report For NAHLN Laboratory Testing April 13, 2013 through June 4, 2014 Biological Accessions State TN TN TX TX TX TX TX TX TX UT UT WI WI WI WI WI WY Week Tested Positive % Pos Total 4/6/2014 4/20/2014 Total 4/6/2014 4/13/2014 4/20/2014 4/27/2014 5/4/2014 5/11/2014 5/25/2014 Total 4/6/2014 4/20/2014 Total 4/13/2014 4/20/2014 4/27/2014 5/18/2014 5/25/2014 Total 5/18/2014 Total 150 2 1 3 4 2 3 1 2 3 2 17 1 10 1 2 1 1 1 1 6 1 1 0 6.67% 0.00% 0.00% 0.00% 25.00%

0.00% 33.33% 0.00% 0.00% 0.00% 0.00% 11.76% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% United States Department of Agriculture 0 1 1 2 0 0 Biological Samples Tested Positive Environmental Samples Tested Positive 494 8 3 11 12 16 23 2 5 9 8 75 1 17 34 2 0 1 0 0 2 1 0 1 7 1 4 1 4 17 1 1 0 8 8 0 0 0 0 0 0 0 1 1 Animal and Plant Health Inspection Service Veterinary Services 52